| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049494 |
| Chromosome: | chromosome 3 |
| Location: | 2732076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161750 | lunapark domain protein involved in vesicle localization | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGATGTCATGTCGTCATCGGTACAGTA |
| Internal bar code: | TCCTAACAGCCGTTCGTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1243 |
| LEAP-Seq percent confirming: | 99.7009 |
| LEAP-Seq n confirming: | 6334 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCAGCAGTGGTACAAGA |
| Suggested primer 2: | TTGCCAGAACCTAATCACCC |