Insertion junction: LMJ.RY0402.049499_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.049499
Chromosome:chromosome 5
Location:1582608
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g243452 (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCCCTCCGCGACGCCTCGGCCCGTTCC
Internal bar code:ACTGGTTTCGATACGCGGCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:102
LEAP-Seq percent confirming:68.1004
LEAP-Seq n confirming:190
LEAP-Seq n nonconfirming:89
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCGCTGATTTCCAGTCA
Suggested primer 2:CTACCCAACTCATGCCCTGT