Insertion junction: LMJ.RY0402.049505_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.049505
Chromosome:chromosome 11
Location:2891854
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g477850 TXC1,TSN1 (1 of 1) K15979 - staphylococcal nuclease domain-containing protein 1 (SND1); Transcriptional coactivator-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGGTCTGGTTGGTGGGGGCGGGCACGT
Internal bar code:CCCTGCGCCGACATCGCTAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:703
LEAP-Seq percent confirming:96.9872
LEAP-Seq n confirming:2189
LEAP-Seq n nonconfirming:68
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATTTAACCTCCAACCCA
Suggested primer 2:AATACTGATCCGTAACGGCG