Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.049516 |
Chromosome: | chromosome_14 |
Location: | 1869014 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g620600 | PHC2 | Cell wall protein pherophorin-C2 | sense | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTAGTTTGGCGTGCGATGCAATCATTCAT |
Internal bar code: | CCACGCGCCAGCACGCGCCACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 600 |
LEAP-Seq percent confirming: | 99.7184 |
LEAP-Seq n confirming: | 2125 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGCATCAGCCACTTGTGAA |
Suggested primer 2: | GCCTTGGAGCAGCAGTAATC |