| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049522 |
| Chromosome: | chromosome 6 |
| Location: | 6276275 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACGCGACCGTAGCGGTGTTGAGAATTG |
| Internal bar code: | ATGAATGAGAGATTGACCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 746 |
| LEAP-Seq percent confirming: | 94.6534 |
| LEAP-Seq n confirming: | 9737 |
| LEAP-Seq n nonconfirming: | 550 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTAGGGGCACTGGTGTG |
| Suggested primer 2: | GTTCATTTGCGGTACGGTCT |