| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049525 |
| Chromosome: | chromosome 11 |
| Location: | 1731704 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467786 | (1 of 1) 1.14.13.15 - Cholestanetriol 26-monooxygenase / Vitamin D(3) 25-hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCAGCCACACAGCAACAGCAGCGGCAC |
| Internal bar code: | GCGGACTACATTGGCTGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 99.0615 |
| LEAP-Seq n confirming: | 6122 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGATAACGCTGGTGGTGT |
| Suggested primer 2: | TGACCCTACTCCTGACCCTG |