Insertion junction: LMJ.RY0402.049554_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.049554
Chromosome:chromosome 10
Location:4198814
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g449950 (1 of 1) PTHR14593:SF5 - WD REPEAT-CONTAINING PROTEIN 11 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTAAACAATCATGAATGTAACCCCAGC
Internal bar code:TACCCGGGGCGTTCCGCGTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:157
LEAP-Seq percent confirming:93.9806
LEAP-Seq n confirming:968
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTGTCACCCTGTCGTTG
Suggested primer 2:GGGCTAACATGACCAGCACT