| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049557 |
| Chromosome: | chromosome 3 |
| Location: | 3808588 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170400 | (1 of 1) PF12579 - Protein of unknown function (DUF3755) (DUF3755) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGAGCCATGCACATGCTTGGCGGTGCT |
| Internal bar code: | CGCCTTGCGTCACTCTCCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 99.8975 |
| LEAP-Seq n confirming: | 1950 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGTGCCTATCATCTTGA |
| Suggested primer 2: | ATGGACCCTTCGTCCTTCTT |