Insertion junction: LMJ.RY0402.049558_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.049558
Chromosome:chromosome 11
Location:1116916
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467679 (1 of 3) PF04982 - HPP family (HPP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTGGCCTCCCGGGCAGCGCCCGTGCGC
Internal bar code:CCAGGTTTCCTGACTCTGATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:362
LEAP-Seq percent confirming:99.6047
LEAP-Seq n confirming:2016
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCATCTTGAGGAACAGCA
Suggested primer 2:CTGAGGGTGCAAGTATGGGT