| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049569 |
| Chromosome: | chromosome 3 |
| Location: | 3301730 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166150 | (1 of 1) IPR015077 - Domain of unknown function DUF1858 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTTGCTGCCCCACTCCCCGCTCACTTG |
| Internal bar code: | TTTGTGATGTACGCGTAAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 99.8478 |
| LEAP-Seq n confirming: | 1968 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGAGAAGGACCAGTGAG |
| Suggested primer 2: | TGGTTACACGAGCGAGAGTG |