| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049580 |
| Chromosome: | chromosome 12 |
| Location: | 3488571 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496100 | FAP149 | Flagellar Associated Protein 149 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACAGTCCTGCTCTGACCTCCTGCCGGT |
| Internal bar code: | TCCGGGTCTAAACCGCCATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 98.5507 |
| LEAP-Seq n confirming: | 680 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTGGCTATGTGCCTGTT |
| Suggested primer 2: | CAGGACGTCTACAAGTCGCA |