Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.049580 |
Chromosome: | chromosome_17 |
Location: | 3972273 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g728700 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCATGAAGGTGGGACTGCGGGGTGTGTGT |
Internal bar code: | GGCTAGGCCGCGGTGGCGTATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 722 |
LEAP-Seq percent confirming: | 99.2851 |
LEAP-Seq n confirming: | 3333 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
Suggested primer 2: | GGTAAGGTGAGGAGCCTTCC |