| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.049580 |
| Chromosome: | chromosome_17 |
| Location: | 3972273 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g728700 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCTGTGATGGTGTGGTCGCGGTAGGCCTT |
| Internal bar code: | CGCGGCCTGCTAACTACCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 99.115 |
| LEAP-Seq n confirming: | 336 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | GGTAAGGTGAGGAGCCTTCC |