| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049595 |
| Chromosome: | chromosome 12 |
| Location: | 5711875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532750 | EEP8 | (1 of 1) K10772 - AP endonuclease 2 (APEX2); Endonuclease/exonuclease/phosphatase family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACATGCACTGATGCTTCCTCTCTGTT |
| Internal bar code: | GCTCAAAAGTAGCCTCCCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 99.1403 |
| LEAP-Seq n confirming: | 2537 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACTTCTAGACAGCAGGG |
| Suggested primer 2: | CATGGTGGACTGAACGTCAC |