Insertion junction: LMJ.RY0402.049609_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.049609
Chromosome:chromosome 9
Location:4109852
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393654 RLS11 RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCACCAGGACGCGCGAAGCACTGTTGC
Internal bar code:CAGGCTCAATTGTGACCAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:719
LEAP-Seq percent confirming:99.3952
LEAP-Seq n confirming:11339
LEAP-Seq n nonconfirming:69
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TAACGTCTTGAGGTGGGGTC
Suggested primer 2:GCGACTGCTAGATAGGCACC