| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049624 |
| Chromosome: | chromosome 1 |
| Location: | 4026857 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026150 | (1 of 3) 3.4.24.59 - Mitochondrial intermediate peptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGAATGCGTTAGGCGCGCCGCAGTCT |
| Internal bar code: | GTCTTGGTCTAATTCCTACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 103 |
| LEAP-Seq percent confirming: | 94.1026 |
| LEAP-Seq n confirming: | 367 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGGGTCTAAAGCCGATG |
| Suggested primer 2: | TTAAGCATAGGGTTGGGTGG |