Insertion junction: LMJ.RY0402.049637_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.049637
Chromosome:chromosome 16
Location:7121600
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g680342 FAP110 Flagellar Associated Protein 110; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCACGGGGTGGTGGGGAGGTTTGGGCT
Internal bar code:AATGGCGCCCAAAGTCGGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:228
LEAP-Seq percent confirming:99.4614
LEAP-Seq n confirming:554
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAATAGGATTGGCTCAGGGG
Suggested primer 2:GTCGTCCATCAACTCCAGGT