Insertion junction: LMJ.RY0402.049654_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.049654
Chromosome:chromosome 17
Location:3344126
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g723450 FAP13 WD-repeat Flagellar Associated Protein 13; (1 of 1) IPR001680//IPR007111//IPR017986//IPR020472//IPR027417 - WD40 repeat // NACHT nucleoside triphosphatase // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCAGACGCGCACGGTTGTGTCACTCCGG
Internal bar code:TATCTTGAGTGTCTGTATTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:197
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:187
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACCACCTCGTTTGTCCTTG
Suggested primer 2:GGCGGCTGGATAATCAGATA