| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049674 |
| Chromosome: | chromosome 7 |
| Location: | 804303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318276 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGAGACAGTGTGAGGCAGCCAAGCATC |
| Internal bar code: | AAGTTGTCATGTATAAATTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 391 |
| LEAP-Seq percent confirming: | 99.5618 |
| LEAP-Seq n confirming: | 10905 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCCGTGGAGCTATGTG |
| Suggested primer 2: | GTCGGGTTGTCAGTGACCTT |