| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049688 |
| Chromosome: | chromosome 12 |
| Location: | 1370299 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488350 | (1 of 2) K00464 - all-trans-8'-apo-beta-carotenal 15,15'-oxygenase (diox1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCATCAATGGGCAAGCGGCAGGATGAAT |
| Internal bar code: | TCGGTCGGACCGGGTCGCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.7416 |
| LEAP-Seq n confirming: | 3474 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCAACTGAGTGCAAGA |
| Suggested primer 2: | GTAGCGGTGGATGAGTGGTT |