Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.049691 |
Chromosome: | chromosome 9 |
Location: | 7209964 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g412350 | (1 of 1) PTHR16557:SF4 - 2-OXOGLUTARATE-DEPENDENT DIOXYGENASE FAMILY PROTEIN-RELATED | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTTGGGGCATGACGCGCCAGCGGTTC |
Internal bar code: | AGGCGGCGACAGAACGGGATAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 396 |
LEAP-Seq percent confirming: | 99.4843 |
LEAP-Seq n confirming: | 5787 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGAGATGGGCAGTTGTAGGA |
Suggested primer 2: | CTCATTATCTCCTGCGCCTC |