Insertion junction: LMJ.RY0402.049692_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.049692
Chromosome:chromosome 1
Location:4283851
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g028777 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTCTGCTATTTGCGCCTCCGGACTCAAA
Internal bar code:TGGATGTAAGGTAGTACTTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:329
LEAP-Seq percent confirming:99.4271
LEAP-Seq n confirming:3471
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGACACGACATCACACG
Suggested primer 2:ACCGACCAGAACTCAACACC