| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049726 |
| Chromosome: | chromosome 12 |
| Location: | 1825485 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511952 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCACTGCTCTTGAAGGAAACTGAAATG |
| Internal bar code: | GTGTTGCCTGTCGGGACCATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.1543 |
| LEAP-Seq n confirming: | 469 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAATGAGTTGACCCTCG |
| Suggested primer 2: | GATATCAGCGCCAGCCTTAG |