| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049727 |
| Chromosome: | chromosome 8 |
| Location: | 1475731 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364700 | (1 of 7) PF00076//PF14259 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) // RNA recognition motif (a.k.a. RRM, RBD, or RNP domain) (RRM_6) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCCCATCGACGTAAAGGAGCGCGAGCT |
| Internal bar code: | GAGTTGTACGGTCGACCCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.4787 |
| LEAP-Seq n confirming: | 3435 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGGGTTTGTTCCCAGTAG |
| Suggested primer 2: | TAACACGAGACGTCAGTGGC |