| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049734 |
| Chromosome: | chromosome 10 |
| Location: | 1443577 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428550 | DTS1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATGGCAACTGGCACTACATTGCAGTCC |
| Internal bar code: | TAAGCTGGCAGGACCGCGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 98.8247 |
| LEAP-Seq n confirming: | 1009 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCGGAAATGTTGTGTG |
| Suggested primer 2: | TAGCGGCGTGACAAGTAGTG |