| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049747 |
| Chromosome: | chromosome 6 |
| Location: | 6373813 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292216 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAACCATCTTGCAGTAACACGCACAGGG |
| Internal bar code: | AGAAAGCGGGGCGAGTCTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 95.7055 |
| LEAP-Seq n confirming: | 936 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCAATCGTTCTGCTCTG |
| Suggested primer 2: | AACAGTGCAGTCCCAAAACC |