Insertion junction: LMJ.RY0402.049751_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.049751
Chromosome:chromosome 17
Location:542983
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g699600 SBP2,SEBP2 (1 of 2) 3.1.3.37 - Sedoheptulose-bisphosphatase / Sedoheptulose-1,7-bisphosphatase; putative Sugar bisphosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAATCTCACGAAACCCCCAGCACGTCAG
Internal bar code:GGCGGGTAGGTAATTGGTGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:662
LEAP-Seq percent confirming:99.3555
LEAP-Seq n confirming:925
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTATGGCTGCTCTGATGC
Suggested primer 2:CTTTTGTGATCGAGGTGGGT