| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049779 |
| Chromosome: | chromosome 9 |
| Location: | 3633595 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390319 | (1 of 1) PTHR10663:SF42 - PROTEIN BOI2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTAGCGCAAAGCCAGCACAGCGTTGGGA |
| Internal bar code: | CGATCAGCTAGGGAGGCCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 92.3241 |
| LEAP-Seq n confirming: | 9935 |
| LEAP-Seq n nonconfirming: | 826 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCAGAACGACTTCCTGC |
| Suggested primer 2: | GTAGAGGCGGTCTATGCGAG |