Insertion junction: LMJ.RY0402.049798_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.049798
Chromosome:chromosome 6
Location:1642381
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g260950 (1 of 1) K12483 - EH domain-containing protein 1 (EHD1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTCGATAACCCCTTCCCGTGAGGGGTTG
Internal bar code:TCTGTGCGCGCTGTAGATGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:674
LEAP-Seq percent confirming:99.3268
LEAP-Seq n confirming:10475
LEAP-Seq n nonconfirming:71
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTGGAACATGTGGCTTC
Suggested primer 2:CCGTGTCCAAGCCATCTATT