Insertion junction: LMJ.RY0402.049829_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.049829
Chromosome:chromosome 11
Location:349072
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467573 LHCA3 light-harvesting chlorophyll-a/b protein of photosystem I (Type III); (1 of 1) PTHR21649//PTHR21649:SF14 - CHLOROPHYLL A/B BINDING PROTEIN // PSI TYPE III CHLOROPHYLL A/B-BINDING PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTTGCGTTTGGCAAAGTGATCTCGCTCA
Internal bar code:AGGCGAAACTTGATCGTCAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1148
LEAP-Seq percent confirming:99.1879
LEAP-Seq n confirming:855
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGAACCACTTGATGTTGGTG
Suggested primer 2:GACATGGATACCAGCACACG