| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049831 |
| Chromosome: | chromosome_16 |
| Location: | 3520253 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g677382 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTTGGCTTCGTGTCAAGCCTATCCGTGC |
| Internal bar code: | TCTCGCGTCGGACAGCGTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 98.5115 |
| LEAP-Seq n confirming: | 33752 |
| LEAP-Seq n nonconfirming: | 510 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTATCTGCTTCCCGTGT |
| Suggested primer 2: | GGCACAACAGCAGAAGAACA |