| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049854 |
| Chromosome: | chromosome 6 |
| Location: | 1870135 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263100 | (1 of 2) PF10263 - SprT-like family (SprT-like) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCTCAAACTCGTCCATGGGCTCTACAC |
| Internal bar code: | CACGGCGAGGGCGGTAAACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 96.0588 |
| LEAP-Seq n confirming: | 3266 |
| LEAP-Seq n nonconfirming: | 134 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCTTTCAAACAACGGC |
| Suggested primer 2: | GCCTTTCTGCCTGAACACTC |