Insertion junction: LMJ.RY0402.049946_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.049946
Chromosome:chromosome 1
Location:3820763
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g024750 STK (1 of 1) PF00059//PF00069//PF07714//PF14295 - Lectin C-type domain (Lectin_C) // Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) // PAN domain (PAN_4); Serine/threonine protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCTGATGTGCACTGGTCGATCTGGGGTG
Internal bar code:CACAATGGCGCCTAGGATCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:678
LEAP-Seq percent confirming:99.6118
LEAP-Seq n confirming:6929
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGGTGTCCACCAAGAACT
Suggested primer 2:TTCATCAGTGCAAGTCCGAG