Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.049946 |
Chromosome: | chromosome_1 |
Location: | 3820763 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g024750 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GATCTGATGTGCACTGGTCGATCTGGGGTG |
Internal bar code: | CACAATGGCGCCTAGGATCGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 678 |
LEAP-Seq percent confirming: | 99.6118 |
LEAP-Seq n confirming: | 6929 |
LEAP-Seq n nonconfirming: | 27 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTGGTGTCCACCAAGAACT |
Suggested primer 2: | TTCATCAGTGCAAGTCCGAG |