Insertion junction: LMJ.RY0402.049978_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.049978
Chromosome:chromosome 17
Location:4871915
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g734250 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACACCCCCAACCCCCTCCACCAAGTCCA
Internal bar code:ACTCACATTGAAATACGTAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:332
LEAP-Seq percent confirming:60.6715
LEAP-Seq n confirming:759
LEAP-Seq n nonconfirming:492
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AATTGAATTTGACCACCCCA
Suggested primer 2:AAGTCGTTACCGAATGTGGG