| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049998 |
| Chromosome: | chromosome 11 |
| Location: | 2452284 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475450 | (1 of 1) K11863 - Ataxin-3 (ATXN3, MJD) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAATAGTACACCGTATAATTCAAACCA |
| Internal bar code: | GCCGTGCGAACAGGCTGGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1024 |
| LEAP-Seq percent confirming: | 99.1491 |
| LEAP-Seq n confirming: | 2913 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGGGCACTTTTGCTAGG |
| Suggested primer 2: | TATTTCACCGGCATGAGACA |