| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050005 |
| Chromosome: | chromosome 3 |
| Location: | 8798046 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205921 | (1 of 5) 3.1.13.5 - Ribonuclease D / RNase D | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCTGAAACCTTGAGCGGGCTAGAGCGG |
| Internal bar code: | TAATGGATGCGTAGTGAGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 98.895 |
| LEAP-Seq n confirming: | 3580 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTCATTCTTCCCACAGC |
| Suggested primer 2: | CTTGTCAACGGCCATAGGTT |