| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050128 |
| Chromosome: | chromosome 14 |
| Location: | 546242 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611650 | ALO2,ALO1 | D-arabinono-1%252C4-lactone oxidase; (1 of 2) 1.1.3.37 - D-arabinono-1,4-lactone oxidase / D-arabinono-gamma-lactone oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAGGGCAGGATAGCGATGCAGGAAGCG |
| Internal bar code: | CGGCGTACATAAATTCTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 99.6466 |
| LEAP-Seq n confirming: | 846 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTAACACACAGGTCGTGG |
| Suggested primer 2: | CTTTGCTTCCATCTGCACAA |