| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050128 |
| Chromosome: | chromosome 14 |
| Location: | 1187413 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615900 | FBT3 | (1 of 4) PTHR31585:SF0 - FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC; putative folate/pteridine transporter 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCCCAGCTCCTTGTGATGAGCTTTGG |
| Internal bar code: | TACGGAAACGTATCATCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1039 |
| LEAP-Seq percent confirming: | 98.5493 |
| LEAP-Seq n confirming: | 1019 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTGTTGTGCTGCGTTAT |
| Suggested primer 2: | GATAGGGCAGTGAGCTGAGG |