| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050133 |
| Chromosome: | chromosome 1 |
| Location: | 588285 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003463 | (1 of 1) K08735 - DNA mismatch repair protein MSH2 (MSH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGATGGGTTTGGGTTGGGTTGGGGGTGG |
| Internal bar code: | CTTCTTGCTTTCCGTAGATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 103 |
| LEAP-Seq percent confirming: | 98.8636 |
| LEAP-Seq n confirming: | 174 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACCTCCTCCTCC |
| Suggested primer 2: | TACAGACGGACACCCAACAA |