| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050165 |
| Chromosome: | chromosome 12 |
| Location: | 432692 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493700 | ZXE2 | Putative zeaxanthin epoxidase; (1 of 4) PF01494//PF13450 - FAD binding domain (FAD_binding_3) // NAD(P)-binding Rossmann-like domain (NAD_binding_8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCCCCGCGCGGCGCCGTGGGCGTCGGG |
| Internal bar code: | GTGCACGGCGACGCCTCGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 74.8287 |
| LEAP-Seq n confirming: | 7973 |
| LEAP-Seq n nonconfirming: | 2682 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCATGTATGGACGGCTA |
| Suggested primer 2: | GTGAGGCGGTAGATGGTGTT |