| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050176 |
| Chromosome: | chromosome 3 |
| Location: | 3172830 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165215 | ATG7 | (1 of 1) 2.7.7.49//2.7.7.7//2.7.7.80//3.1.26.4 - RNA-directed DNA polymerase / Revertase // DNA-directed DNA polymerase / DNA-dependent DNA polymerase // Molybdopterin-synthase adenylyltransferase // Ribonuclease H / RNase H; E1 activating enzyme for ATG8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGCCTGTGAACTTCTGCCCACCCACCC |
| Internal bar code: | CACTCGATTCGACACTTGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 99.6753 |
| LEAP-Seq n confirming: | 921 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATGAGAACCTTCCCGCT |
| Suggested primer 2: | ATGGGCGTCATAAAGTTTGC |