| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.050178 |
| Chromosome: | chromosome 6 |
| Location: | 4303596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278256 | RPN8 | (1 of 1) K03038 - 26S proteasome regulatory subunit N8 (PSMD7, RPN8); 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATACAATCGCACTCCCCACACCAAGCCAG |
| Internal bar code: | GTCGCACCGTCTCCCAGTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1079 |
| LEAP-Seq percent confirming: | 99.7414 |
| LEAP-Seq n confirming: | 22369 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACCACACG |
| Suggested primer 2: | GGAGGACTGCAATACGGTGT |