| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050188 |
| Chromosome: | chromosome 1 |
| Location: | 113889 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000800 | CGL136 | Conserved in the Green Lineage | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAAGCCACATTGTTCACGCACACTTGCA |
| Internal bar code: | CTCGGCCAGATAGGAGATATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.3827 |
| LEAP-Seq n confirming: | 483 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGACGGACCACAAAAG |
| Suggested primer 2: | GTCTTTTGAGCGGCGATAAG |