| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050191 |
| Chromosome: | chromosome 6 |
| Location: | 3558518 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278116 | (1 of 1) IPR008136 - CinA, C-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCCCCACCCGCGCGCTTGCCACGTCTC |
| Internal bar code: | GTTAGATGCACAAGCGCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 86.152 |
| LEAP-Seq n confirming: | 10769 |
| LEAP-Seq n nonconfirming: | 1731 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGCGGCTGTAGTAGCA |
| Suggested primer 2: | AAACCAACCAAGTCTGGACG |