| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050204 |
| Chromosome: | chromosome 1 |
| Location: | 7494950 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053900 | (1 of 1) K01456 - peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase (E3.5.1.52, NGLY1, PNG1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGACACAAACGATCAAGTCACAGGGCT |
| Internal bar code: | GTCCGACCCCTATCGTCTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1200 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTACTTTCAGCGAGCAC |
| Suggested primer 2: | ATAATTTTGGCCCTGCTGTG |