| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050209 |
| Chromosome: | chromosome 1 |
| Location: | 3109719 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019300 | Nucleotidyl cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCTGCTGCTGCTGCTGCCGTGTCGAG |
| Internal bar code: | GGGATTTGCTTGTGTAGCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 79.0816 |
| LEAP-Seq n confirming: | 620 |
| LEAP-Seq n nonconfirming: | 164 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGCAAGGAAGTGCTGTG |
| Suggested primer 2: | GCAAGAGCAGCCCTAACAAC |