Insertion junction: LMJ.RY0402.050215_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.050215
Chromosome:chromosome 13
Location:3734397
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGAAGACTGCTGAGCACTCCGCATCGCT
Internal bar code:TCGTAAGGACCTGGGCGTGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:676
LEAP-Seq percent confirming:70.6189
LEAP-Seq n confirming:1084
LEAP-Seq n nonconfirming:451
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGAGAGGTCCTTGGAGG
Suggested primer 2:GACTGGTGGACGAGGGACTA