| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050228 |
| Chromosome: | chromosome 11 |
| Location: | 1537274 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467752 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGGTGGTCGATGAGTTCTTAGGCCCCA |
| Internal bar code: | CAGTATTCGTAATGCCGATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.0368 |
| LEAP-Seq n confirming: | 5038 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACACGAGAGCAGTTCA |
| Suggested primer 2: | GACAGCAGCAGTAGCAGCAG |