| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.050267 |
| Chromosome: | chromosome 8 |
| Location: | 1296642 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363750 | (1 of 2) K06268 - serine/threonine-protein phosphatase 2B regulatory subunit (PPP3R, CNB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGCACCAACAAGTTGTTTTGGCTTATG |
| Internal bar code: | TGGTTCTCGGACCTCAGTATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 99.9397 |
| LEAP-Seq n confirming: | 1657 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACATCGTGTGGCGTTCAT |
| Suggested primer 2: | CATGACCCTGATTGTCGTTG |