| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050279 |
| Chromosome: | chromosome 12 |
| Location: | 3561309 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513450 | TUH1,TUH | Zeta tubulin; (1 of 1) PTHR11588//PTHR11588:SF6 - TUBULIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGGGCGTCACTTGTCGCAACTTGAGT |
| Internal bar code: | CGTCCGGTGTAATCCGGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1110 |
| LEAP-Seq percent confirming: | 99.6967 |
| LEAP-Seq n confirming: | 9204 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGGAAAACGGGCTATCT |
| Suggested primer 2: | CCTCTCCATGTTTGGGTTTG |